R1701-3

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IF-Cell, IHC-P
Clonality:	Polyclonal

Form:

Liquid

Storage condition:

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Storage buffer:

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration:

1ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

Predicted band size: 82 kDa

Isotype:

IgG

Immunogen:

Recombinant protein within Human Calpain 1 aa 293-519 / 714.

Positive control:

HUVEC, MCF-7, human tonsil tissue, human kidney tissue, 2 kidney tissue.

Subcellular location:

Cytoplasm. Cell membrane.

Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:500
1:50-1:200
1:50-1:200

Uniprot #:

SwissProt: P07384 Human | O35350 Mouse | P97571 Rat

Alternative names:

Ca2 activated neutral protease Calcium activated neutral proteinase Calcium activated neutral proteinase small subunit Calcium dependent protease small subunit 1 Calcium dependent protease small subunit Calcium-activated neutral proteinase 1 Calpain 1 Calpain 1 large subunit Calpain 1, (mu/I) large subunit Calpain mu type Calpain mu-type Calpain regulatory subunit Calpain small subunit 1 Calpain, large polypeptide L1 Calpain-1 catalytic subunit Calpain-1 large subunit CAN1_HUMAN CANP 1 CANP CANP small subunit CANP1 CANPL 1 CANPL1 CAPN 1 CAPN1 Cell proliferation inducing protein 30 Cell proliferation-inducing gene 30 protein Micromolar Calpain Micromolar-calpain Mu Calpain muCANP muCL

	Fig1: Western blot analysis of Calpain 1 on HUVEC cell lysate using anti-Calpain 1 antibody at 1/500 dilution.
	Fig2: ICC staining Calpain 1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig3: ICC staining Calpain 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
	Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.

	Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.
	Fig6: Immunohistochemical analysis of paraffin-embedded 2 kidney tissue using anti-Calpain 1 antibody. Counter stained with hematoxylin.