COX IV Rabbit Polyclonal Antibody
cat.: R1706-1
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 20 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human COX IV aa 46-89. |
| Positive control: | Mouse heart tissue lysate, mouse skeletal muscle tissue lysate, rat heart tissue lysate, rat skeletal muscle tissue lysate, Hela, HepG2, MCF-7, rat skeletal muscle tissue, human colon cancer tissue, human kidney tissue, mouse heart tissue. |
| Subcellular location: | Mitochondrion inner membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:5,000 1:100-1:500 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P13073 Human | P19783 Mouse | P10888 Rat |
| Alternative names: | AL024441 COX 4 COX IV 1 COX IV COX IV-1 Cox4 COX41_HUMAN Cox4a COX4B COX4I1 COX4I2 COX4L2 COXIV Cytochrome c oxidase polypeptide IV Cytochrome c oxidase subunit 4 isoform 1 mitochondrial Cytochrome c oxidase subunit 4 isoform 1, mitochondrial Cytochrome C Oxidase subunit IV Cytochrome c oxidase subunit IV isoform 1 Cytochrome c oxidase subunit IV isoform 2 (lung) Cytochrome c oxydase subunit 4 dJ857M17.2 MGC105470 MGC72016 |
Images
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Fig1:
Western blot analysis of COX IV on different lysates with Rabbit anti-COX IV antibody (R1706-1) at 1/5,000 dilution. Lane 1: Mouse heart tissue lysate Lane 2: Mouse skeletal muscle tissue lysate Lane 3: Rat heart tissue lysate Lane 4: Rat skeletal muscle tissue lysate Lysates/proteins at 15 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1706-1, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 20 kDa Observed band size: 15 kDa |
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Fig2: ICC staining COX IV in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining COX IV in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining COX IV in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-COX IV antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-COX IV antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-COX IV antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-COX IV antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.