Jagged 1 Rabbit Polyclonal Antibody
cat.: R1706-10
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 133 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Jagged 1 aa 90-144 / 1,218. |
| Positive control: | 293T, Hela, HepG2, Raw264.7, rat kidney tissue, mouse kidney tissue. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
IF-Cell IHC-P FC |
1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P78504 Human | Q9QXX0 Mouse | Q63722 Rat |
| Alternative names: | AGS AHD AWS CD 339 CD339 CD339 antigen Headturner hJ1 Htu Jag 1 Jag1 JAG1_HUMAN Jagged 1 Jagged1 (Alagille syndrome) Jagged1 JAGL1 MGC104644 OTTHUMP00000030278 Protein jagged-1 Ser 1 Ser1 Serrate 1 Slalom |
Images
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Fig1:
Immunocytochemistry analysis of Raw264.7 cells labeling Jagged 1 with Rabbit anti-Jagged 1 antibody (R1706-10) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Jagged 1 antibody (R1706-10) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. |
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Fig2: ICC staining Jagged 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Jagged 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Jagged 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Jagged 1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Jagged 1 antibody. Counter stained with hematoxylin. |
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Fig7: Flow cytometric analysis of HepG2 cells with Jagged 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.