IREB2 Rabbit Polyclonal Antibody
cat.: R1706-12
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 105/38 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human IREB2 aa 17-301 / 963. |
| Positive control: | mouse liver tissue lysate, human liver tissue lysate, 293T, A549, HepG2, human kidney tissue, human placenta tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:2000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P48200 Human | Q811J3 Mouse | Q62751 Rat |
| Alternative names: | ACO3 FLJ23381 IRE BP2 IRE-BP 2 IREB 2 IREB2 IREB2_HUMAN Iron regulatory protein 2 iron responsive element binding protein 2 Iron-responsive element-binding protein 2 IRP 2 IRP2 IRP2AD OTTHUMP00000185022 |
Images
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Fig1:
Western blot analysis of IREB2 on mouse liver lysate with Rabbit anti-IREB2 antibody (R1706-12) at 1/2,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1706-12, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 105 kDa Observed band size: 105 kDa |
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Fig2:
Western blot analysis of IREB2 on human liver lysate with Rabbit anti-IREB2 antibody (R1706-12) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1706-12, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 105 kDa Observed band size: 105 kDa |
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Fig3: ICC staining IREB2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining IREB2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: ICC staining IREB2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IREB2 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IREB2 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.