NSUN4 Rabbit Polyclonal Antibody
cat.: R1706-16
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 43 kDa
Isotype: IgG
Immunogen: Recombinant protein within N-terminal human NSUN4.
Positive control: MCF-7, 293, A549, HepG2, human liver cancer tissue, human kidney tissue, mouse testis tissue.
Subcellular location: Mitochondrion.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:1,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q96CB9 Human | Q9CZ57 Mouse
Alternative names: 5 methylcytosine rRNA methyltransferase NSUN4 EC=2.1.1.- NOL1/NOP2/Sun domain family member 4 NOL1/NOP2/Sun domain family, member 4 NOP2/Sun domain family, member 4 Nsun4 NSUN4_HUMAN Putative methyltransferase NSUN4 RP4-603I14.2 Shtap Sperm head and tail associated protein
Images
R1706-16_1.jpg Fig1: Western blot analysis of NSUN4 on different cell lysates using anti-NSUN4 antibody at 1/1,000 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: 293
R1706-16_2.jpg Fig2: ICC staining NSUN4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-16_3.jpg Fig3: ICC staining NSUN4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-16_4.jpg Fig4: ICC staining NSUN4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-16_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.
R1706-16_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.
R1706-16_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.