Neurofilament Rabbit Polyclonal Antibody
cat.: R1706-17
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 112 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Neurofilament aa 279-415. |
| Positive control: | N2A, rat brain tissue, mouse brain tissue. |
| Subcellular location: | Cytoplasm, Intermediate filament. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:1000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P12036 Human | P19246 Mouse | P16884 Rat |
| Alternative names: | 200 kDa neurofilament protein CMT2CC Nefh Neurofilament heavy polypeptide 200kDa Neurofilament heavy polypeptide Neurofilament triplet H protein NF H NF-H NFH NFH_HUMAN |
Images
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Fig1: Western blot analysis of Neurofilament on mouse brain tissue lysate using anti-Neurofilament antibody at 1/500 dilution. |
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Fig2: ICC staining Neurofilament in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Neurofilament antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Neurofilament antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.