Tyrosine Hydroxylase Rabbit Polyclonal Antibody
cat.: R1706-18
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Tyrosine Hydroxylase aa 1-50 / 528. |
| Positive control: | Rat brain tissue, mouse brain tissue, PC-12 cell lysates. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:1,000 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P07101 Human | P24529 Mouse | P04177 Rat |
| Alternative names: | Dystonia 14 DYT14 DYT5b EC 1.14.16.2 OTTHUMP00000011225 OTTHUMP00000011226 ple Protein Pale TH The TY3H_HUMAN TYH Tyrosine 3 hydroxylase Tyrosine 3 monooxygenase Tyrosine 3-hydroxylase Tyrosine 3-monooxygenase Tyrosine hydroxylase |
Images
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Fig1: ICC staining Tyrosine Hydroxylase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining Tyrosine Hydroxylase in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Tyrosine Hydroxylase antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tyrosine Hydroxylase antibody. Counter stained with hematoxylin. |
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Fig5:
Western blot analysis of Tyrosine Hydroxylase on PC-12 cell lysate with Rabbit anti-Tyrosine Hydroxylase antibody (R1706-18) at 1/1,000 dilution. Lysates/proteins at 10 µg/Lane. Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1706-18, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/100,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 58 kDa Observed band size: 58 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.