ALDH1A1 Rabbit Polyclonal Antibody
cat.: R1706-2
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: Predicted band size: 55 kDa
Isotype: IgG
Immunogen: Recombinant protein within human Aldh1A1 aa 1-160/501.
Positive control: 2 liver tissue lysate, 2 kidney tissue lysate, 293T, A431, HepG2, human liver tissue, human lung tissue, human kidney tissue, 2 colon tissue, A549.
Subcellular location: Cytoplasm.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC

1:500-1:1000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P00352 Human | P24549 Mouse | P51647 Rat
Alternative names: Acetaldehyde dehydrogenase 1 AHD2 AL1A1_HUMAN ALDC Aldehyde dehydrogenase 1 family member A1 Aldehyde dehydrogenase 1 soluble Aldehyde dehydrogenase 1A1 Aldehyde dehydrogenase Aldehyde dehydrogenase cytosolic Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase liver cytosolic ALDH 1 ALDH 1A1 ALDH class 1 ALDH, liver cytosolic ALDH-E1 ALDH1 A1 ALDH1 ALDH11 ALDH1A1 ALHDII cytosolic epididymis luminal protein 12 epididymis luminal protein 9 epididymis secretory sperm binding protein Li 53e HEL-S-53e MGC2318 PUMB1 RALDH 1 RalDH1 Retinal dehydrogenase 1
Images
R1706-2_1.jpg Fig1: Western blot analysis of Aldh1A1 on 2 liver (1) and 2 kidney (2) tissue lysates using anti-Aldh1A1 antibody at 1/1,000 dilution.
R1706-2_2.jpg Fig2: ICC staining Aldh1A1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-2_3.jpg Fig3: ICC staining Aldh1A1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-2_4.jpg Fig4: ICC staining Aldh1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-2_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin.
R1706-2_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin.
R1706-2_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin.
R1706-2_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded 2 colon tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin.
R1706-2_9.jpg Fig9: Flow cytometric analysis of A549 cells with Aldh1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody;black).
R1706-2_10.jpg Fig10: Flow cytometric analysis of HepG2 cells with Aldh1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.