Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | Predicted band size: 55 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human Aldh1A1 aa 1-160/501. |
Positive control: | 2 liver tissue lysate, 2 kidney tissue lysate, 293T, A431, HepG2, human liver tissue, human lung tissue, human kidney tissue, 2 colon tissue, A549. |
Subcellular location: | Cytoplasm. |
Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1000 1:50-1:200 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: P00352 Human | P24549 Mouse | P51647 Rat |
Alternative names: | Acetaldehyde dehydrogenase 1 AHD2 AL1A1_HUMAN ALDC Aldehyde dehydrogenase 1 family member A1 Aldehyde dehydrogenase 1 soluble Aldehyde dehydrogenase 1A1 Aldehyde dehydrogenase Aldehyde dehydrogenase cytosolic Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase liver cytosolic ALDH 1 ALDH 1A1 ALDH class 1 ALDH, liver cytosolic ALDH-E1 ALDH1 A1 ALDH1 ALDH11 ALDH1A1 ALHDII cytosolic epididymis luminal protein 12 epididymis luminal protein 9 epididymis secretory sperm binding protein Li 53e HEL-S-53e MGC2318 PUMB1 RALDH 1 RalDH1 Retinal dehydrogenase 1 |
Fig1: Western blot analysis of Aldh1A1 on 2 liver (1) and 2 kidney (2) tissue lysates using anti-Aldh1A1 antibody at 1/1,000 dilution. | |
Fig2: ICC staining Aldh1A1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining Aldh1A1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining Aldh1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. | |
Fig8: Immunohistochemical analysis of paraffin-embedded 2 colon tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
Fig9: Flow cytometric analysis of A549 cells with Aldh1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody;black). | |
Fig10: Flow cytometric analysis of HepG2 cells with Aldh1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |