ALDH1A1 Rabbit Polyclonal Antibody
cat.: R1706-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Aldh1A1 aa 1-160/501. |
| Positive control: | 2 liver tissue lysate, 2 kidney tissue lysate, 293T, A431, HepG2, human liver tissue, human lung tissue, human kidney tissue, 2 colon tissue, A549. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P00352 Human | P24549 Mouse | P51647 Rat |
| Alternative names: | Acetaldehyde dehydrogenase 1 AHD2 AL1A1_HUMAN ALDC Aldehyde dehydrogenase 1 family member A1 Aldehyde dehydrogenase 1 soluble Aldehyde dehydrogenase 1A1 Aldehyde dehydrogenase Aldehyde dehydrogenase cytosolic Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase liver cytosolic ALDH 1 ALDH 1A1 ALDH class 1 ALDH, liver cytosolic ALDH-E1 ALDH1 A1 ALDH1 ALDH11 ALDH1A1 ALHDII cytosolic epididymis luminal protein 12 epididymis luminal protein 9 epididymis secretory sperm binding protein Li 53e HEL-S-53e MGC2318 PUMB1 RALDH 1 RalDH1 Retinal dehydrogenase 1 |
Images
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Fig1: Western blot analysis of Aldh1A1 on 2 liver (1) and 2 kidney (2) tissue lysates using anti-Aldh1A1 antibody at 1/1,000 dilution. |
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Fig2: ICC staining Aldh1A1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Aldh1A1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Aldh1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
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Fig8: Immunohistochemical analysis of paraffin-embedded 2 colon tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
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Fig9: Flow cytometric analysis of A549 cells with Aldh1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody;black). |
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Fig10: Flow cytometric analysis of HepG2 cells with Aldh1A1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.