ALDH1A1 Rabbit Polyclonal Antibody
cat.: R1706-2
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC(Intra) |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Aldh1A1 aa 1-160/501. |
| Positive control: | A549 (Human lung adenocarcinoma cell) cell lysate, HT-29 (Human colorectal adenocarcinoma cell) cell lysate, mouse liver tissue lysate, mouse lung tissue lysate, rat liver tissue lysate, rat lung tissue lysate, A549, human lung tissue, human liver tissue, human kidney tissue, human colon tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:5,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P00352 Human | P24549 Mouse | P51647 Rat |
| Alternative names: | Acetaldehyde dehydrogenase 1 AHD2 AL1A1_HUMAN ALDC Aldehyde dehydrogenase 1 family member A1 Aldehyde dehydrogenase 1 soluble Aldehyde dehydrogenase 1A1 Aldehyde dehydrogenase Aldehyde dehydrogenase cytosolic Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase liver cytosolic ALDH 1 ALDH 1A1 ALDH class 1 ALDH, liver cytosolic ALDH-E1 ALDH1 A1 ALDH1 ALDH11 ALDH1A1 ALHDII cytosolic epididymis luminal protein 12 epididymis luminal protein 9 epididymis secretory sperm binding protein Li 53e HEL-S-53e MGC2318 PUMB1 RALDH 1 RalDH1 Retinal dehydrogenase 1 |
Images
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Fig1:
Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody (R1706-2) at 1/5,000 dilution. Lane 1: A549 (Human lung adenocarcinoma cell) cell lysate (15 µg/Lane) Lane 2: HT-29 (Human colorectal adenocarcinoma cell) cell lysate (15 µg/Lane) Lane 3: Mouse liver tissue lysate (30 µg/Lane) Lane 4: Mouse lung tissue lysate (30 µg/Lane) Lane 5: Rat liver tissue lysate (30 µg/Lane) Lane 6: Rat lung tissue lysate (30 µg/Lane) Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1706-2, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 50 kDa |
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Fig2:
Application: Immunocytochemistry (IF-cell) Species: Human Sample: A549 (Human lung adenocarcinoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Triton X-100, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 1 hour at room temperature. Antibody dilution buffer: 1% BSA in PBST. Primary antibody: R1706-2, 1/100, overnight at 4℃. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 45 minutes at room temperature. Counterstain: Beta tubulin (HA601187, Red), 1/100, overnight at 4℃. The nuclear counterstain was DAPI (Blue). |
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Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
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Fig7:
Application: Flow Cytometry (Intra) Species: Human Sample: A549 (Human lung adenocarcinoma cell) Fixation: 4% Paraformaldehyde, 15 minutes at room temperature. Permeabilization: 0.1% Tween-20, 15 minutes at room temperature. Blocking: 1% BSA + 10% normal goat serum, 15 minutes at room temperature. Antibody dilution buffer: 1x PBS. Primary antibody: R1706-2 (1/1,000, Red) compared with Rabbit IgG Isotype Control (HA722127, Green), 15 minutes at room temperature. Secondary antibody: Goat Anti-Rabbit IgG (iFluor™ 488, HA1121), 15 minutes at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.