DDB1 Rabbit Polyclonal Antibody
cat.: R1706-21
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 127 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human DDB1 aa 151-560 / 1,140. |
| Positive control: | Mouse colon tissue lysate, PC-12, Siha, A549, SH-SY5Y, SK-Br-3, K562, human breast, human kidney, rat brain. |
| Subcellular location: | Nucleus. Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1000 1:100-1:500 1:100-1:500 1:50-1:100 |
| Uniprot #: | SwissProt: Q16531 Human | Q3U1J4 Mouse | Q9ESW0 Rat |
| Alternative names: | Damage specific DNA binding protein 1 Damage-specific DNA-binding protein 1 DDB 1 DDB p127 subunit Ddb1 DDB1_HUMAN DDBa DNA damage binding protein 1 DNA damage-binding protein 1 DNA damage-binding protein a HBV X-associated protein 1 UV damaged DNA binding factor UV damaged DNA binding protein 1 UV DDB 1 UV DDB1 UV-damaged DNA-binding factor UV-damaged DNA-binding protein 1 UV-DDB 1 X associated protein 1 XAP 1 XAP-1 XAP1 Xeroderma pigmentosum group E complementing protein Xeroderma pigmentosum group E-complementing protein XPCe XPE XPE BF XPE binding factor XPE-BF XPE-binding factor |
Images
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Fig1:
Western blot analysis of DDB1 on different cell lysate using anti-DDB1 antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse colon tissue Lane 2: PC-12 Lane 3: Siha |
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Fig2: ICC staining DDB1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining DDB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining DDB1 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDB1 antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-DDB1 antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-DDB1 antibody. Counter stained with hematoxylin. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-DDB1 antibody (R1706-21) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1706-21) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9: Flow cytometric analysis of K562 cells with DDB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.