Tyrosinase Rabbit Polyclonal Antibody
cat.: R1706-4
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 70 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Tyrosinase aa 51-100 / 529.
Positive control: B16F1, Hela, human urethral melanoma tissue, A431.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:200-1:500
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P14679 Human
Alternative names: ATN CMM8 LB24 AB LB24-AB Monophenol monooxygenase OCA1 OCA1A OCAIA Oculocutaneous albinism IA SHEP3 SK29 AB SK29-AB Tumor rejection antigen AB TYR TYRO_HUMAN tyrosinase (oculocutaneous albinism IA) Tyrosinase
Images
R1706-4_1.jpg Fig1: Western blot analysis of Tyrosinase on B16F1 cell lysates using anti- Tyrosinase antibody at 1/1,000 dilution.
R1706-4_2.jpg Fig2: ICC staining Tyrosinase in B16F1 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-4_3.jpg Fig3: ICC staining Tyrosinase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-4_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human urethral melanoma tissue using anti-Tyrosinase antibody. Counter stained with hematoxylin.
R1706-4_5.jpg Fig5: Flow cytometric analysis of A431 cells with Tyrosinase antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.