Tim3 Rabbit Polyclonal Antibody
cat.: R1706-6
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 40 kDa/70 kDa (additional band) |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human Tim3 aa 51-100 / 301. |
| Positive control: | Hela, mouse spleen tissue, PC-3M cell lysate, human spleen tissue, human breast cancer tissue. |
| Subcellular location: | Cell junction, Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: Q8TDQ0 Human | Q8VIM0 Mouse |
| Alternative names: | CD366 FLJ14428 HAVcr-2 Havcr2 HAVR2_HUMAN Hepatitis A virus cellular receptor 2 Kidney injury molecule 3 KIM 3 KIM3 T cell immunoglobulin and mucin domain containing 3 T cell immunoglobulin mucin 3 T-cell immunoglobulin and mucin domain-containing protein 3 T-cell immunoglobulin mucin family member 3 T-cell immunoglobulin mucin receptor 3 T-cell membrane protein 3 Tim 3 TIM-3 TIM3 TIMD-3 TIMD3 |
Images
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Fig1:
Western blot analysis of Tim3 on different lysates using anti-Tim3 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: Mouse spleen Lane 3: PC-3M |
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Fig2: ICC staining Tim3 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Tim3 antibody. Counter stained with hematoxylin. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti- Tim3 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti- Tim3 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.