G6PD Rabbit Polyclonal Antibody
cat.: R1706-7
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 59 kDa
Isotype: IgG
Immunogen: Recombinant protein within human G6PD aa 218-404.
Positive control: A549, Hela, MCF-7, PC-12, mouse spleen tissue lysate, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse testis tissue.
Subcellular location: Cytoplasm. Nucleus. Membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P

1:1,000-1:2,000
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: P11413 Human | Q00612 Mouse | P05370 Rat
Alternative names: G6PD G6PD_HUMAN G6PD1 G6pdx Glucose 6 phosphate 1 dehydrogenase Glucose 6 phosphate dehydrogenase Glucose 6 phosphate dehydrogenase, G6PD Glucose-6-phosphate 1-dehydrogenase MET19 POS10 Zwf1p
Images
R1706-7_1.jpg Fig1: Western blot analysis of G6PD on different lysates using anti-G6PD antibody at 1/1,000 dilution.
Positive control:
Lane 1: A549 Lane 2: Hela Lane 3: MCF-7 Lane 4: PC-12 Lane 5: Mouse spleen
R1706-7_2.jpg Fig2: ICC staining G6PD in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-7_3.jpg Fig3: ICC staining G6PD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-7_4.jpg Fig4: ICC staining G6PD in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
R1706-7_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-G6PD antibody. Counter stained with hematoxylin.
R1706-7_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-G6PD antibody. Counter stained with hematoxylin.
R1706-7_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-G6PD antibody. Counter stained with hematoxylin.
R1706-7_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-G6PD antibody. Counter stained with hematoxylin.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.