G6PD Rabbit Polyclonal Antibody
cat.: R1706-7
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 59 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human G6PD aa 218-404. |
| Positive control: | A549, Hela, MCF-7, PC-12, mouse spleen tissue lysate, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse testis tissue. |
| Subcellular location: | Cytoplasm. Nucleus. Membrane. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P11413 Human | Q00612 Mouse | P05370 Rat |
| Alternative names: | G6PD G6PD_HUMAN G6PD1 G6pdx Glucose 6 phosphate 1 dehydrogenase Glucose 6 phosphate dehydrogenase Glucose 6 phosphate dehydrogenase, G6PD Glucose-6-phosphate 1-dehydrogenase MET19 POS10 Zwf1p |
Images
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Fig1: ICC staining G6PD in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig2: ICC staining G6PD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining G6PD in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-G6PD antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-G6PD antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-G6PD antibody. Counter stained with hematoxylin. |
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Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-G6PD antibody. Counter stained with hematoxylin. |
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Fig8:
Western blot analysis of G6PD on different lysates with Rabbit anti-G6PD antibody (R1706-7) at 1/1,000 dilution. Lane 1: MCF7 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: PC-12 cell lysate Lane 4: NIH/3T3 cell lysate Lane 5: mouse spleen cell lysate Lane 6: mouse testis cell lysate Lane 7: rat spleen cell lysate Lysates/proteins at 15 µg/Lane. Exposure time: 4 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1706-7, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 59 kDa Observed band size: 59 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.