Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human, Mouse, Rat |
Applications: | WB, IF-Cell, IHC-P |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Protein A affinity purified. |
Molecular weight: | 59 kDa |
Isotype: | IgG |
Immunogen: | Recombinant protein within human G6PD aa 218-404. |
Positive control: | A549, Hela, MCF-7, PC-12, mouse spleen tissue lysate, human tonsil tissue, human colon cancer tissue, human spleen tissue, mouse testis tissue. |
Subcellular location: | Cytoplasm. Nucleus. Membrane. |
Recommended Dilutions:
WB IF-Cell IHC-P |
1:1,000-1:2,000 1:50-1:200 1:50-1:200 |
Uniprot #: | SwissProt: P11413 Human | Q00612 Mouse | P05370 Rat |
Alternative names: | G6PD G6PD_HUMAN G6PD1 G6pdx Glucose 6 phosphate 1 dehydrogenase Glucose 6 phosphate dehydrogenase Glucose 6 phosphate dehydrogenase, G6PD Glucose-6-phosphate 1-dehydrogenase MET19 POS10 Zwf1p |
Fig1:
Western blot analysis of G6PD on different lysates using anti-G6PD antibody at 1/1,000 dilution. Positive control: Lane 1: A549 Lane 2: Hela Lane 3: MCF-7 Lane 4: PC-12 Lane 5: Mouse spleen |
|
Fig2: ICC staining G6PD in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig3: ICC staining G6PD in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. | |
Fig4: ICC staining G6PD in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-G6PD antibody. Counter stained with hematoxylin. | |
Fig6: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-G6PD antibody. Counter stained with hematoxylin. | |
Fig7: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-G6PD antibody. Counter stained with hematoxylin. | |
Fig8: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-G6PD antibody. Counter stained with hematoxylin. |