Fatty Acid Synthase Rabbit Polyclonal Antibody
cat.: R1706-8
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 273 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human Fatty Acid Synthase aa 801-1050. |
| Positive control: | A549, MCF-7, Hela, human prostate tissue, human breast cancer tissue. |
| Subcellular location: | Cytoplasm. Melanosome. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2,000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P49327 Human | P19096 Mouse | P12785 Rat |
| Alternative names: | [Acyl-carrier-protein] S acetyltransferase [Acyl-carrier-protein] S malonyltransferase 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase 3-oxoacyl-[acyl-carrier-protein] reductase 3-oxoacyl-[acyl-carrier-protein] synthase Enoyl-[acyl-carrier-protein] reductase FAS FAS_HUMAN FASN Fatty acid synthase MGC14367 MGC15706 OA 519 Oleoyl-[acyl-carrier-protein] hydrolase SDR27X1 Short chain dehydrogenase/reductase family 27X member 1 |
Images
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Fig1:
All lanes: Western blot analysis of Fatty Acid Synthase with anti-Fatty Acid Synthase antibody (R1706-8) at 1:1,000 dilution. Lane 1: Wild-type Hela whole cell lysate. Lane 2: FASN knockdown Hela whole cell lysate. R1706-8 was shown to specifically react with FASN in wild-type Hela cells. Weakened band was observed when FASN knockdown samples were tested. Wild-type and FASN knockdown samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-FASN antibody (R1706-8, 1/1,000) and Anti-HSP90 antibody (ET1605-56, 1/10,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig2: Western blot analysis of Fatty Acid Synthase on A549 cell lysate using anti-Fatty Acid Synthase antibody at 1/1,000 dilution. |
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Fig3: ICC staining Fatty Acid Synthase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: ICC staining Fatty Acid Synthase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin. |
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Fig6: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.