ALDH1A1 Rabbit Polyclonal Antibody
cat.: R1706-9
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 55 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant full length protein of human Aldh1A1. |
| Positive control: | Mouse liver tissue lysate, human liver tissue lysate, A549, HepG2, rat epididymis tissue, human pancreas tissue. |
| Subcellular location: | Cytoplasm. |
| Recommended Dilutions:
WB IF-Cell IHC-P |
1:500-1:2000 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: P00352 Human | P24549 Mouse | P51647 Rat |
| Alternative names: | Acetaldehyde dehydrogenase 1 AHD2 AL1A1_HUMAN ALDC Aldehyde dehydrogenase 1 family member A1 Aldehyde dehydrogenase 1 soluble Aldehyde dehydrogenase 1A1 Aldehyde dehydrogenase Aldehyde dehydrogenase cytosolic Aldehyde dehydrogenase family 1 member A1 Aldehyde dehydrogenase liver cytosolic ALDH 1 ALDH 1A1 ALDH class 1 ALDH, liver cytosolic ALDH-E1 ALDH1 A1 ALDH1 ALDH11 ALDH1A1 ALHDII cytosolic epididymis luminal protein 12 epididymis luminal protein 9 epididymis secretory sperm binding protein Li 53e HEL-S-53e MGC2318 PUMB1 RALDH 1 RalDH1 Retinal dehydrogenase 1 |
Images
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Fig1:
Western blot analysis of ALDH1A1 on different lysates with Rabbit anti-ALDH1A1 antibody (R1706-9) at 1/2,000 dilution. Lane 1: Hep G2 (Human hepatocellular carcinoma cell) cell lysate (15 µg/Lane) Lane 2: K-562 (Human chronic myelogenous leukemia cell) cell lysate (15 µg/Lane) Lane 3: HT-29 (Human colorectal adenocarcinoma cell) (15 µg/Lane) Lane 4: Mouse liver tissue lysate (30 µg/Lane) Lane 5: Rat liver tissue lysate (30 µg/Lane) Exposure time: 10 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: R1706-9, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 55 kDa Observed band size: 55 kDa |
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Fig2: ICC staining Aldh1A1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig3: ICC staining Aldh1A1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Aldh1A1 antibody. Counter stained with hematoxylin. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.