| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 3G1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 21 kDa |
| Isotype: | IgG1 |
| Immunogen: | peptide |
| Positive control: | 293T, human pancreas tissue. |
| Subcellular location: | Cytoplasm cytosol nucleoplasm |
| Recommended Dilutions:
WB IP IF IHC-P |
1:100-1:1,000 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) 1:50-500 1:50-500 |
| Uniprot #: | SwissProt: Q13541 Human |
| Alternative names: | 4E-BP1 4EBP1 4EBP1_HUMAN BP 1 eIF4E binding protein 1 eIF4E-binding protein 1 Eif4ebp1 Eukaryotic translation initiation factor 4E-binding protein 1 PHAS-I PHASI Phosphorylated heat- and acid-stable protein regulated by insulin 1 |
|
Fig1: Western blot analysis of 4E-BP1 expression in non-transfected(A) and mouse 4E-BP1 transfected(B) 293T whole cell lysates. |
|
Fig2: Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of glandular cells. |