| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 2A1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 56 kDa |
| Isotype: | IgG1 |
| Immunogen: | peptide |
| Positive control: | Hela, MCF7, Langerhans, glandular, human pancreas tissue. |
| Subcellular location: | Cytoplasm, Nucleus, Cell membrane |
| Recommended Dilutions:
WB IP IF IHC-P |
1:100-1:1,000 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) 1:50-500 1:50-500 |
| Uniprot #: | SwissProt: P31751 Human |
| Alternative names: | AKT Akt2 AKT2_HUMAN HIHGHH Murine thymoma viral (v-akt) homolog 2 murine thymoma viral (v-akt) homolog-2 Oncogene AKT2 protein kinase B beta PKB PKB beta PKBB PKBBETA PRKBB Protein kinase Akt 2 Protein kinase Akt-2 Protein kinase B beta RAC beta rac protein kinase beta RAC-BETA RAC-beta serine/threonine-protein kinase RAC-PK-beta RACbeta v akt murine thymoma viral oncogene homolog 2 V-AKT murine thymoma viral oncogene homolog 2 |
|
Fig1: Western blot analysis of Akt2 expression in MCF7 whole cell lysate. |
|
Fig2: Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining (A).Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing granular cytoplasmic staining of Islets of Langerhans and glandular cells (B). |