c-Myc Mouse Monoclonal Antibody [6G6]
cat.: RT1149
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Monkey |
| Applications: | WB, IP, IF, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 6G6 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 67kDa |
| Isotype: | IgG1 |
| Immunogen: | peptide |
| Positive control: | Jurkat, COS |
| Subcellular location: | Nucleus |
| Recommended Dilutions:
WB IP IF IHC-P |
1:100-1:1,000 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) 1:50-1:500 1:50-1:500 FCM: 1 μg per 1 x 106 cells |
| Uniprot #: | SwissProt: P01106 Human |
| Alternative names: | AU016757 Avian myelocytomatosis viral oncogene homolog bHLHe39 c Myc Cellular myelocytomatosis oncogene Class E basic helix-loop-helix protein 39 MGC105490 MRTL Myc Myc protein Myc proto oncogene protein Myc proto-oncogene protein myc-related translation/localization regulatory factor MYC_HUMAN Myc2 myca MYCC Myelocytomatosis oncogene a Myelocytomatosis oncogene Niard Nird oncogene c-Myc Oncogene Myc OTTHUMP00000158589 OTTHUMP00000227763 Proto-oncogene c-Myc Protooncogene homologous to myelocytomatosis virus RNCMYC Transcription factor p64 Transcriptional regulator Myc-A V-Myc avian myelocytomatosis viral oncogene homolog v-myc myelocytomatosis viral oncogene homolog (avian) zc-myc |
Images
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Fig1: c-Myc Antibody: RT1149. Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal human colon showing intense nuclear staining. |
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Fig2: c-Myc Antibody: RT1149. Western blot analysis of c-Myc expression in HeLa (A), Jurkat (B) and K-562 (C) whole cell lysates. |
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Fig3: c-Myc Antibody AF680: RT1149 AF680. Direct near-infrared western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B). Blocked with UltraCruz® Blocking Reagent: sc-516214. |
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Fig4: c-Myc Antibody: RT1149. Immunofluorescence staining of methanol-fixed COS cells transfected with c-Myc fusion protein showing cytoplasmic staining. |
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Fig5: c-Myc Antibody: RT1149. Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear and cytoplasmic staining of squamous epithelial cells. |
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Fig6: c-Myc Antibody: RT1149. Near-infrared western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B). Blocked with UltraCruz® Blocking Reagent: sc-516214. Detection reagent used: m-IgGκ BP-CFL 680: sc-516180. |
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Fig7: c-Myc Antibody AF790: RT1149 AF790. Direct near-infrared western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B). Blocked with UltraCruz® Blocking Reagent: sc-516214. |
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Fig8: c-Myc Antibody HRP: RT1149 HRP. Direct western blot analysis of c-Myc expression in COS whole cell lysates prepared from non-transfected cells (A) and c-Myc fusion protein transfected cells (B). |
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Fig9: c-Myc Antibody: RT1149. Immunoperoxidase staining of formalin fixed, paraffin-embedded human appendix tissue showing cytoplasmic staining of glandular cells and nuclear and cytoplasmic staining of lymphoid cells. |
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Fig10: c-Myc Antibody: RT1149. Western blot analysis of whole cell lysates prepared from COS cells transfected with a c-Myc fusion protein (A) and non-transfected (B) cells. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.