RT1159

Product Type:	Mouse monoclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IP, IF, IHC-P, ELISA
Clonality:	Monoclonal

Form:

Liquid

Storage condition:

Store at +4℃

Storage buffer:

1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Concentration:

2ug/ul

Purification:

Immunogen affinity purified.

Molecular weight:

70-72kDa

Isotype:

IgG

Immunogen:

peptide

Positive control:

RAW 264.7, NIH/3T3, human gall bladder tissue.

Subcellular location:

Microsome membrane, Endoplasmic reticulum membrane

Recommended Dilutions:
  WB
  IP
  IF
  IHC-P
  ELISA

1:100-1:1000
1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
1:50-1:500
1:50-1:500
1:30-1:3000

Uniprot #:

SwissProt: P35354 Human

Alternative names:

COX 2 COX-2 COX2 Cyclooxygenase 2 Cyclooxygenase 2b Cyclooxygenase Cyclooxygenase-2 Cyclooxygenase2 EC 1.14.99.1 fj02a10 Glucocorticoid-regulated inflammatory cyclooxygenase Glucocorticoid-regulated inflammatory Prostaglandin G/H synthase GRIPGHS hCox 2 Macrophage activation-associated marker protein P71/73 OTTHUMP00000033524 PES-2 PGG/HS PGH synthase 2 PGH2_HUMAN PGHS 2 PGHS-2 PGHS2 PHS 2 PHS II PHS2 Prostaglandin endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) Prostaglandin endoperoxide synthase 2 Prostaglandin G/H synthase 2 Prostaglandin G/H synthase 2 precursor Prostaglandin G/H synthase and cyclooxygenase Prostaglandin G/H synthase Prostaglandin H2 synthase 2 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase) Prostaglandin-endoperoxide synthase 2 PTGS2 ptgs2a TIS10 TIS10 protein unp1239 wu:fj02a10

	Fig1: Western blot analysis of Cox-2 expression in untreated (A), LPS treated (B) and LPS and Hydrocortisone treated (C) RAW 264.7 whole cell lysates. Note decreased LPS induction of Cox-2 expression in lane C.expression in lane C.
	Fig2: Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing membrane and nuclear envelope localization (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic staining of glandular cells (B).