| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP |
| Clonality: | Monoclonal |
| Clone number: | 4G3 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 185kDa |
| Isotype: | IgG1 |
| Immunogen: | Amino acid residues surrounding Threonine 686 of Neu of human origin. |
| Positive control: | A431, PMA, LPA, Ceramide, Bradykinin, Bombesin. |
| Subcellular location: | Cytoplasm, Cell membrane, Nucleus |
| Recommended Dilutions:
WB IP |
1:100-1:1,000 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) |
| Uniprot #: | SwissProt: P04626 Human |
| Alternative names: | Receptor tyrosine-protein kinase erbB-2 ERBB2 Receptor tyrosine-protein kinase erbB-2 HER2 Receptor tyrosine-protein kinase erbB-2 MLN19 Receptor tyrosine-protein kinase erbB-2 NEU |
|
Fig1: Western blot analysis of Neu phosphorylation in serum starved A431 (A), and serum starved A431 treated for 15 minutes with PMA (B), LPA (C), Ceramide (D), Bradykinin (E) and Bombesin (F) whole cell lysates. |