Iba1 Recombinant Mouse Monoclonal Antibody [8G8]
cat.: RT1316
| Product Type: | Recombinant Mouse monoclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat, Cynomolgus monkey, Pig |
| Applications: | WB, IHC-P, FC, IP, IHC-Fr, IF-Cell, IF-Tissue |
| Clonality: | Monoclonal |
| Clone number: | 8G8 |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein. |
| Positive control: | Mouse brain tissue, mouse hippocampus tissue, rat brain tissue, RAW264.7, C6, human PBL whole cell lysates. |
| Subcellular location: | Cytoplasm, cytoskeleton. |
| Recommended Dilutions:
WB IHC-P IHC-Fr IF-Cell FC IP IF-Tissue |
1:1,000 1:500-1:2000 1:1,000 1:200 1:200-1:1,000 Use at an assay dependent concentration 1:1,000 |
| Uniprot #: | SwissProt: P55008 Human |
| Alternative names: | AIF 1 AIF-1 Aif1 AIF1 protein AIF1_HUMAN Allograft inflammatory factor 1 Allograft inflammatory factor 1 splice variant G allograft inflammatory factor-1 splice variant Hara-1 balloon angioplasty responsive transcription BART 1 G1 G1 putative splice variant of allograft inflamatory factor 1 IBA 1 IBA1 interferon gamma responsive transcript Interferon responsive transcript 1 interferon responsive transcript factor 1 Ionized calcium binding adapter molecule 1 Ionized calcium-binding adapter molecule 1 ionized calcium-binding adapter molecule IRT 1 IRT1 Microglia response factor MRF1 Protein g1 |
Images
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Fig1:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required |
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Fig2:
Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/1,000 (Iba1, RT1316, green); 1/500 (MAP2, HA723025, red) Antigen retrieval: Not required |
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Fig3:
Application: IHC-Fr Species: Rat Site: Cerebellum Sample: Frozen section Antibody concentration: 1/1,000 (Iba1, RT1316, green); 1/500 (MAP2, HA723025, red) Antigen retrieval: Not required |
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Fig4:
Application: IHC-Fr Species: Mouse Site: Cerebral cortex Sample: Frozen section Antibody concentration: 1/1,000 Antigen retrieval: Not required |
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Fig5:
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Iba1 antibody (RT1316) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (RT1316) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig6:
Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-Iba1 antibody (RT1316) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (RT1316) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig7:
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Iba1 antibody (RT1316) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (RT1316) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig8:
Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-Iba1 antibody (RT1316) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (RT1316) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig9:
Immunocytochemistry analysis of RAW264.7 cells labeling Iba1 with Mouse anti-Iba1 antibody (RT1316) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Iba1 antibody (RT1316) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. |
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Fig10:
Immunocytochemistry analysis of C6 cells labeling Iba1 with Mouse anti-Iba1 antibody (RT1316) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Iba1 antibody (RT1316) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution. |
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Fig11:
Application: IF-Tissue Species: Mouse Site: brain Sample: Paraffin-embedded section Antibody concentration: 1/1,000 |
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Fig12: Western blot analysis of Iba1 on human PBL whole cell lysate using anti-Iba1 antibody at 1/1,000 dilution. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.