| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 1G1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃. |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 130 kDa |
| Isotype: | IgG1 |
| Immunogen: | Amino acids 785-815 within an internal region of JAK1 of mouse origin. |
| Subcellular location: | Endomembrane system. |
| Recommended Dilutions:
WB IP IF IHC-P |
1:100-1:1,000 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) 1:50-1:500 1:50-1:500 |
| Uniprot #: | SwissProt: P23458 Human | P52332 Mouse |
| Alternative names: | JAK 1 JAK 1A JAK 1B JAK-1 JAK1 JAK1_HUMAN JAK1A JAK1B Janus kinase 1 (a protein tyrosine kinase) Janus kinase 1 JTK3 Tyrosine protein kinase JAK 1 Tyrosine protein kinase JAK1 Tyrosine-protein kinase JAK1 |
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Fig1: JAK1 Antibody: RT1341. Western blot analysis of JAK1 expression in NIH/3T3 (A), C6 (B), A-431 (C), MCF7 (D) and HeLa (E) whole cell lysates. |
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Fig2: JAK1 Antibody: RT1341. Immunoperoxidase staining of formalin-fixed, paraffin-embedded mouse kidney tissue showing membrane and cytoplasmic localization. |
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Fig3: JAK1 Antibody: RT1341. Immunofluorescence staining of methanol-fixed Jurkat cells showing membrane localization. |
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Fig4: JAK1 Antibody HRP: RT1341 HRP. Direct western blot analysis of JAK1 expression in SUP-T1 (A), NTERA-2 cl.D1 (B), Jurkat (C), HuT 78 (D), TF-1 (E) and T-47D (F) whole cell lysates |
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Fig5: JAK1 Antibody: RT1341. Western blot analysis of JAK1 expression in SUP-T1 (A), TK-1 (B) and NTERA-2 cl.D1 (C) whole cell lysates. |
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Fig6: JAK1 Antibody: RT1341. Western blot analysis of JAK1 expression in Jurkat (A), MOLT-4 (B) and HuT 78 (C) whole cell lysates. |
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Fig7: JAK1 Antibody: RT1341. Western blot analysis of JAK1 expression in Jurkat (A) and TF-1 (B) whole cell lysates. |
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Fig8: JAK1 Antibody: RT1341. Western blot analysis of JAK1 expression in non-transfected (A) and truncated mouse JAK1 transfected (B) 293T whole cell lysates. |