| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 1G1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 200kDa |
| Isotype: | IgG1 |
| Immunogen: | Amino acids 1158-1345 mapping at the C-terminus of the Flk-1 of mouse origin. |
| Positive control: | 293T, HUV-EC-C |
| Subcellular location: | Cytoplasm, Nucleus, Secreted, Cell junction Endoplasmic reticulum, Cell membrane, Cytoplasmic vesicle, Early endosome |
| Recommended Dilutions:
WB IP IF IHC-P |
1:100-1:1,000 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) 1:50-1:500 1:50-1:500 |
| Uniprot #: | SwissProt: P35968 Human |
| Alternative names: | CD309 CD309 antigen EC 2.7.10.1 Fetal liver kinase 1 FLK-1 FLK1 FLK1 mouse homolog of Kdr Kinase insert domain receptor (a type III receptor tyrosine kinase) Kinase insert domain receptor KRD1 Ly73 Protein tyrosine kinase receptor FLK1 Protein-tyrosine kinase receptor flk-1 soluble VEGFR2 Tyrosine kinase growth factor receptor Vascular endothelial growth factor receptor 2 VEGFR 2 VEGFR VEGFR-2 VEGFR2 VGFR2_HUMAN |
|
Fig1: Western blot analysis of Flk-1 expression in non-transfected (A) and mouse Flk-1 transfected (B) 293T whole cell lysates. |
|
Fig2: Western blot analysis of Flk-1 expression in VEGF-treated HUV-EC-C whole cell lysate. |