| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IP, IF, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 395/345kDa |
| Isotype: | IgG |
| Immunogen: | Amino acids 2641-2940 mapping at the C-terminus of Ki-67 of human origin. |
| Positive control: | MCF7, Ki67. |
| Subcellular location: | Nucleus, Chromosome |
| Recommended Dilutions:
WB IP IF IHC-P |
1:100-1:1,000 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) 1:50-1:500 1:50-1:500 |
| Uniprot #: | SwissProt: P46013 Human |
| Alternative names: | Antigen identified by monoclonal Ki 67 Antigen identified by monoclonal Ki-67 Antigen KI-67 Antigen KI67 Antigen Ki67 KI67_HUMAN KIA Marker of proliferation Ki-67 MIB 1 MIB MKI67 PPP1R105 Proliferation marker protein Ki-67 Proliferation related Ki 67 antigen Protein phosphatase 1 regulatory subunit 105 RP11-380J17.2 |
|
Fig1: Western blot analysis of Ki-67 expression in MCF7 nuclear extract. |
|
Fig2: Nuclear Ki67 in mouse mammary gland (60X microscopic magnification in oil). Dilution 1:50 in dilution buffer (0.05% BSA in PBS) Blocking: 0.1% BSA in PBS at room temp. |