| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 67 kDa |
| Isotype: | IgG |
| Immunogen: | Amino acids 401-490 mapping near the C-terminus of Lamin B1 of human origin. |
| Positive control: | Hela, Jurkat, Y79, human heart muscle tissue. |
| Subcellular location: | Nucleus inner membrane |
| Recommended Dilutions:
WB IP IF IHC-P |
1:100-1:1,000 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate) 1:50-1:500 1:50-1:500 |
| Uniprot #: | SwissProt: P20700 Human |
| Alternative names: | ADLD lamin B1 Lamin-B1 LMN LMN2 LMNB Lmnb1 LMNB1_HUMAN MGC111419 OTTHUMP00000159218 |
|
Fig1: Western blot analysis of Lamin B1 expression in Jurkat (A) and Y79 (B) whole cell lysates. . |
|
Fig2: Immunoperoxidase staining of formalin fixed, paraffin-embedded human heart muscle tissue showing nuclear envelope and cytoplasmic staining of myocytes (A). Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear envelope localization (B). . |