MDM2 Mouse Monoclonal Antibody [3G2]
cat.: RT1382
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 3G2 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 55/90 kDa |
| Isotype: | IgG1 |
| Immunogen: | Amino acids 154-167 of MDM2 of human origin. |
| Positive control: | U-2OS, A-673, RAW264.7, human breast carcinoma tissue. |
| Subcellular location: | Cytoplasm, Nucleus |
| Recommended Dilutions:
WB IP IF IHC-P |
1:100-1:1,000 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) 1:50-1:500 1:50-1:500 |
| Uniprot #: | SwissProt: Q00987 Human |
| Alternative names: | ACTFS Double minute 2 protein E3 ubiquitin-protein ligase Mdm2 Hdm 2 Hdm2 HDMX MDM 2 MDM2 MDM2 oncogene E3 ubiquitin protein ligase Mdm2 p53 E3 ubiquitin protein ligase homolog Mdm2 transformed 3T3 cell double minute 2 p53 binding protein (mouse) binding protein 104kDa MDM2_HUMAN MDM2BP Mouse Double Minute 2 MTBP Murine Double Minute Chromosome 2 Oncoprotein Mdm2 p53 Binding Protein Mdm2 p53-binding protein Mdm2 Ubiquitin protein ligase E3 Mdm2 Ubiquitin protein ligase E3 Mdm2 |
Images
|
Fig1: Western blot analysis of MDM2 expression in U-2OS (A), A-673 (B) and RAW 264.7 (C) whole cell lysates. |
|
Fig2: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue (A). Immunofluorescence staining of methanol-fixed rat embryo fibroblasts showing nuclear localization (B). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.