RT1456

Product Type:	Mouse monoclonal IgG1, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IP, IF, IHC-P
Clonality:	Monoclonal
Clone number:	2G1

Form:

Liquid

Storage condition:

Store at +4℃

Storage buffer:

1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.

Concentration:

2ug/ul

Purification:

Protein A affinity purified.

Molecular weight:

70kDa

Isotype:

IgG1

Immunogen:

A sequence containing Ser 434 phosphorylated p70 S6 kinase α of human origin.

Positive control:

HEK293, NIH/3T3, human esophagus tissue.

Subcellular location:

Cytoplasm, Nucleus

Recommended Dilutions:
  WB
  IP
  IF
  IHC-P

1:100-1:1,000
1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
1:50-1:500
1:50-1:500

Uniprot #:

SwissProt: P23443 Human

Alternative names:

70 kDa ribosomal protein S6 kinase 1 KS6B1_HUMAN p70 alpha P70 beta 1 p70 ribosomal S6 kinase alpha p70 ribosomal S6 kinase beta 1 p70 S6 kinase alpha P70 S6 Kinase p70 S6 kinase alpha 1 p70 S6 kinase alpha 2 p70 S6K p70 S6K-alpha p70 S6KA p70(S6K) alpha p70(S6K)-alpha p70-alpha p70-S6K 1 p70-S6K P70S6K P70S6K1 p70S6Kb PS6K Ribosomal protein S6 kinase 70kDa polypeptide 1 Ribosomal protein S6 kinase beta 1 Ribosomal protein S6 kinase beta-1 Ribosomal protein S6 kinase I RPS6KB1 S6K S6K-beta-1 S6K1 Serine/threonine kinase 14 alpha Serine/threonine-protein kinase 14A STK14A

	Fig1: Western blot analysis of p70 S6 kinase α phosphorylation in untreated (A,D), insulin treated (B,E) and insulin treated and lambda protein phosphatase treated (C,F) HEK293 whole cell lysates. Antibodies tested include p-p70 S6 kinase α (2G1) (A,B,C) and p70 S6 kinase α (D,E,F).
	Fig2: Immunoperoxidase staining of formalin fixed, paraffin-embedded human esophagus tissue showing nuclear staining of squamous epithelial cells.