| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF |
| Clonality: | Monoclonal |
| Clone number: | 2G1 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | ERK 1: 44 kDa, ERK 2: 42 kDa |
| Isotype: | IgG1 |
| Immunogen: | Amino acids 325-345 of ERK 1 of rat origin. |
| Positive control: | A-431, rat cerebrum tissue. |
| Subcellular location: | Cytoplasm, Nucleus |
| Recommended Dilutions:
WB IP IF |
1:100-1:1,000 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) 1:50-1:500 |
| Uniprot #: | SwissProt: P28482 Human |
| Alternative names: | ERK 2 ERK-2 ERT1 Extracellular Signal Regulated Kinase 2 Extracellular signal-regulated kinase 2 MAP kinase 1 MAP kinase 2 MAP kinase isoform p42 MAPK 1 MAPK 2 Mapk1 MAPK2 Mitogen-activated protein kinase 1 Mitogen-activated protein kinase 2 MK01_HUMAN P38 P40 P41 p42-MAPK P42MAPK PRKM1 PRKM2 protein kinase mitogen-activated 1 protein kinase mitogen-activated 2 protein tyrosine kinase ERK2 |
|
Fig1: Western blot analysis of ERK 1/2 expression in rat cerebrum tissue extract. |
|
Fig2: Immunofluorescence staining of A-431 cells showing cytoplasmic staining. |