| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IP, IF, IHC-P |
| Clonality: | Monoclonal |
| Clone number: | 4G5 |
| Form: | Liquid |
| Storage condition: | Store at +4℃ |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 2ug/ul |
| Purification: | Protein A affinity purified. |
| Molecular weight: | 48kDa |
| Isotype: | IgG1 |
| Immunogen: | Amino acids 26-214 of SCF of human origin. |
| Positive control: | MCF7, COLO320DM, rat brain tissue, mouse brain tissue, human colon tissue, human lung tissue, human small intestine tissue |
| Subcellular location: | Cytoplasm, Secreted, Cell membrane |
| Recommended Dilutions:
WB IP IF IHC-P |
1:200-1:2,000 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate) 1:50-1:500 1:50-1:500 |
| Uniprot #: | SwissProt: P21583 Human |
| Alternative names: | C kit ligand C-kit ligand Ckit ligand DCUA DFNA69 DKFZp686F2250 familial progressive hyperpigmentation 2 FPH2 FPHH KIT ligand Kitl KITLG KL 1 KL1 Mast cell growth factor MGF MGF stem cell factor SCF SCF_HUMAN SF SHEP7 sKITLG Soluble KIT ligand Steel factor steel mouse homolog of Stem cell factor Stem cell factor precursor |
|
Fig1: Western blot analysis of SCF expression in MCF7 (A) and COLO 320DM (B) whole cell lysates and rat brain (C), mouse brain (D), human colon (E) and human lung (F) tissue extracts. |
|
Fig2: Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing membrane and extracellular staining of glandular cells (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human small intestine tissue showing cytoplasmic staining of goblet cells (B). |