PPAR gamma Mouse Monoclonal Antibody [E-8]
cat.: RT1668
| Product Type: | Mouse monoclonal IgG1, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, IP |
| Clonality: | Monoclonal |
| Clone number: | E-8 |
| Form: | Liquid |
| Storage condition: | Store at 4℃. |
| Storage buffer: | 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. |
| Concentration: | 0.2ug/ul |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG1 |
| Immunogen: | specific for an epitope mapping between amino acids 486-505 at the C-terminus of PPARγ of human origin (identical to corresponding mouse sequence) |
| Positive control: | Jurkat cell lysate, U-937 cell lysate, THP-1 cell lysate, Mouse ovary tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, mouse colon tissue, rat colon tissue. |
| Subcellular location: | Nucleus, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P IP |
1:1,000 1:50-1:200 1:1,000 |
| Uniprot #: | SwissProt: P37231 Human | P37238 Mouse | O88275 Rat |
| Alternative names: | CIMT1 GLM1 NR1C3 Nuclear receptor subfamily 1 group C member 3 OTTHUMP00000185032 OTTHUMP00000185036 Peroxisome proliferator activated nuclear receptor gamma variant 1 Peroxisome proliferator activated receptor gamma 1 Peroxisome Proliferator Activated Receptor gamma Peroxisome proliferator-activated receptor gamma PPAR gamma PPAR-gamma PPARG PPARG_HUMAN PPARG1 PPARG2 PPARgamma |
Images
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Fig1:
Western blot analysis of PPAR gamma on different lysates with Mouse anti-PPAR gamma antibody (RT1668) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: U-937 cell lysate Lane 3: THP-1 cell lysate Lane 4: Mouse ovary tissue lysate Lane 5: Mouse liver tissue lysate Lane 6: Rat liver tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 58 kDa Observed band size: 58/50 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (RT1668) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-PPAR gamma antibody (RT1668) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (RT1668) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3:
Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-PPAR gamma antibody (RT1668) at 1/50 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (RT1668) at 1/50 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.